Sentence examples for media were loaded from inspiring English sources

Cell lysate and media were loaded on a 10% Tris-HCl gel (Bio-Rad Criterion).

In total, 15 20 μl of conditioned media were loaded per lane.

After filling the lower chamber with complete media, 2 × 104 HCC cells in 0.5 mL serum-free media were loaded onto the upper chamber.

Cells (1.5 × 10) in 100 μl serum-free media were loaded onto the pre-soaked Roche E-plates and measurements were taken at intervals of 10 min [ 26].

In all, 20  μg of protein from the cell layer and 25  μg of protein from the cell media were loaded onto a 12% SDS polyacrylamide gel.

Equal volumes of conditioned media were loaded and processed for western blotting as previously described above except that primary polyclonal rabbit anti-human IL-18 antibody was used (Santa Cruz biotechnology, Santa Cruz, CA, USA).

His election campaign enjoyed all the usual advantages of incumbency and state media is loaded in his favour.

The media are loaded with war-related stories.

Cell suspension (200 μl) in serum-free media was loaded into each transwell insert, and 750 μl of medium with 10% FBS was provided in the lower chamber; 4 × 104 MDA-MB231/BT-549 cells were loaded in each transwell.

The conditioned media was loaded onto a 10-ml column of anti-Flag M2 affinity gel and washed with 100 ml of TBS buffer (10 mM Tris HCl, 150 mM NaCl, pH 7.4) to remove the unbound proteins.

Fresh or conditioned media (CM) were loaded to the two reservoirs on both sides of each chamber and cells were imaged for 3 h using a Nikon Swept-Field laser confocal microscope (Nikon Instruments, New York, NY).