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Clones that grew on selection media were isolated and examined for the strength and specificity of their interaction with psoriasin.
Briefly, 24 and 48 hours posttreatment/posttransfection, the cells and media were isolated and permeabilized with 70% ethanol.
Exosomes released by LAMA84 CML cells after a 24-hour culture period in presence of FBS previously ultracentrifuged (vesicle free media) were isolated from conditioned culture medium by differential centrifugation, as previously described [ 6, 8].
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Total RNA from mammary tissue, purified, and resuscitated cells cultured with induction media was isolated with ice-cold TriZol solution (Invitrogen).
Briefly, cultures were diluted with sterilized media, individual colonies were isolated under a microscope, and transferred to fresh, sterile media for growth.
After manual picking, colonies were grown overnight in standard LB-kanamycin media, and plasmids were isolated by alkaline lysis method using PerfectPrep (Eppendorf) or Montage (Millipore) kits.
In solid media, only Thermoactinomyces, bacteria were isolated.
Although mutants generated in this way are all beneficial in the selective media on which they were isolated, the assumption is that they represent a random collection of fitness effects (deleterious, neutral, and potentially beneficial) in other unrelated environments.
EVs were isolated from media bathing either apical or basolateral RPE surfaces, and two subpopulations of small EVs including exosomes, and dense EVs, were purified and processed for proteomic profiling.
After 12 28 h of incubation, plastic adherent cells were isolated with media change and those were termed hASCs.
Both strains of " Spiribacter" were isolated on media for heterotrophic aerobic microbes M19-400 on pyruvate and yeast extract and UAH-SP71 on pyruvate, dextrose and peptone).
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CEO of Professional Science Editing for Scientists @ prosciediting.com