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The media were inoculated with 0.4 mL of a standardized bacterial inoculation.
Both media were inoculated with B. amyloliquefaciens BS-20 and cultivated for 48 h.
For the production of enzymes (protease and chitinase), two different culture media were inoculated with AC001 conidia.
The growth media were inoculated with 4%% spore suspension and incubated at 40 °C at 160 rpm for 5 days.
The media were inoculated with a grown preculture of the respective bacterial strain, constituting 10% of the total volume.
When media were inoculated with 2 g L−1 of yeast, all strains were able to completely consume the available sugars in less than 14 h.
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To enhance seedling quality, the seedbeds or container media are inoculated with specific microorganisms that form symbiotic relationships with the seedlings.
The conical flask containing the above sterilized media was inoculated with fungal spores and incubated at orbital shaker at 29°C for 84 hours at 140 rpm.
50 µl of each last bacterial suspension in 10 mM MgSO4 (see Bacterial strain and growth media) was inoculated in 800 µl of Sucrose Tryptone medium (ST) containing 20 g L−1 sucrose and 5 g L−1 tryptone.
Briefly, 50 µl of each last bacterial suspension in 10 mM MgSO4 (see Bacterial strain and growth media) was inoculated in 96-well plates containing 1 mL 1/10 diluted 869-rich medium complemented with 50 mg mL−1 tryptophan and incubated at 30 °C for 4 days at 150 rpm in the dark.
Briefly, 50 µl of each last bacterial suspension in 10 mM MgSO4 (see Bacterial strain and growth media) was inoculated in 96-well plates containing 800 µl of 284 medium prepared at three different iron concentrations: without iron, with 0.25 µM, and 3 µM Fe(III) citrate.
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