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Media alone and soluble, freshly filtered protein dissolved in media were injected to serve as baseline for our experiments.
For orthotopic assays, 5 × 106 MDA-MB-231 or MCF-7 cells in Matrigel (BD, Biosciences) plus growth media were injected into the mammary fat pads of nude mice (n = 3 4 per group).
Approximately 50,000 cells in 1 µl of neural expansion media were injected over a period of 10 min. The needle was then removed and the incision sealed with veterinary-grade tissue adhesive (Vetbond, 3 M).
Rat hearts were infarcted, and BMSCs or control media were injected into the infarct periphery (n = 34) or infused systemically (n = 30).
Cells in their 9th to 13th in vitro passages at a concentration of 2 × 106 in 0.3 ml culture media were injected into the lateral back wall of female BALB/c (nu/nu) mice at 4 weeks of age (2 mice each per cell system).
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For the 3D cone-beam CTA images, 24 ml contrast media was injected at a flow rate of 3 ml/s, and the injection started 3 s before start of the C-arm rotation and continued during the 5 s of the rotation.
When iodinated radiographic contrast media are injected intravenously or intra-arterially, they pass from the vascular compartment through capillaries into the extracellular space.
For the 2D images, 15 ml contrast media was injected at a flow rate of 3 ml/s, and the frame rate was 2 images/s (Fig. 1).
When radiographic contrast media are injected intravenously or intra-arterially, they pass from the vascular compartment through capillaries into the extracellular space.
Approximately 2 ml non-ionic water soluble contrast media was injected with patient pain appreciation as a guideline on the amount of contrast.
The mean transit time (MTT) in each ROI was determined as ∑(0– ∞) Ct/∑(0– ∞) C, where C is the quantity of contrast medium remaining at the site and t is the time after the contrast media is injected.
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