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At the end of the culture period, the constructs and corresponding media were immediately stored at -70°C before analysis.
At the end of the culture period, all constructs and corresponding media were immediately stored at -80°C prior to analysis.
At the end of the culture period, the constructs and corresponding media were immediately stored at -20°C before biochemical analysis.
At the end of the culture period, the constructs and corresponding media were immediately stored at -20°C prior to biochemical analysis.
After incubation for 24 hours at one percent oxygen, remaining cells were removed from the media by centrifugation and these conditioned media were immediately used for the experiments.
Half of the macrophage-conditioned media were immediately frozen and stored for analysis, and half were placed on fibroblast cultures for 24 hr to obtain co-conditioned media.
Similar(54)
Indeed, the fAβ originally added into the media was immediately and thoroughly internalized by microglia and little did we observe any resecretion in the media (Fig. S1A).
Marcin, whose work most often explores representations of women in the media, was immediately intrigued.
A proposed law, which would have created a Media Authority "for the regulation, development and promotion of an independent and professional media," was immediately shelved.
The media was immediately decanted and the cells fixed with 4% paraformaldehyde.
Media was immediately aspirated, cells were washed in 5 mL of 1X PBS and 10 ml of supplemented DMEM was added and cells were grown for 48 hours.
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