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Conditioned media were generated from cultured IFP and scAT explants, and cells (chondrocytes, synoviocytes) were isolated after sequential enzymatic digestion of the corresponding tissues.
Conditioned media were generated by culturing E14 progenitors at 50,000 cells/ml for 1 week, after which cultures were centrifuged, media removed and filtered to eliminate any remaining cells or debris and the media was frozen in single use aliquots.
Yeast media were generated and used according to (Hoffmann et al, 2003).
Control media were generated in the same way but at normoxic conditions.
For pH-neutralized experiments, media were generated as described above, but then, 1 M NaHCO3 was added to return the pH to that of the control.
To optimize the production medium, 19 new media were generated based on the reference medium from A. terreus[ 4], by varying several components or their concentrations per medium (Tables 2 1).
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I feel responsible for murder and slaughter that's taken place in South Sudan that's not being reported on, partly because there's not as much social media being generated from there.
3D porous media are generated by truncating the gaussian distribution.
The stochastic media are generated by resorting to Poisson random tessellations in 1d slab, 2d extruded, and full 3d geometry.
The presented modeled data points of tight media are generated by altering the pore and throat size distribution of Berea sand network having constant coordination number.
Most of the published data on NP mobility in porous media was generated using water saturated artificial columns frequently made of quartz sand [e.g. 111] while only a few involved natural soil [e.g. 92].
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