Sentence examples for media were gelled from inspiring English sources
All media were gelled with 0.9 % agar.
All media were gelled with 0.9% Difco Bacto-agar (Difco Laboratories, Detroit, MI, USA).
All media were gelled with 0.45% agar and sterilized by autoclaving at 1.4 kg cm−2 for 20 min.
At 7 T, the media/agarose has a T1 of 2952 ± 152 ms and a T2 of 96 ± 1 ms. In a 4 mL 15 × 45 mm glass vial, 2 mL of media/agarose was gelled at room temperature around a 5 mm o.d.
The effect of different treatments (suspended growth with Activated Sludge Process; attached growth with Fixed Film; and combined treatment with Polyvinyl Alcohol Gel media) were analyzed.
For the determination of plasminogen activators, conditioned media were analysed on SDS polyacrylamide gels containing 1 mg ml−1 gelatin and 3 μg ml−1 plasminogen.
Briefly, media were aspirated and the collagen gel was transferred to a centrifuge tube, followed by a brief centrifugation at 4°C.
After the addition of 2× sample buffer, cell media were directly loaded on to gels.
The concentrated media were subjected to cation exchange, gel filtration, and anion exchange chromatography, yielding rhTyrRS of up to 97% purity.
In all, 50 μg of extracted total proteins or proteins of spent media were subjected to SDS-PAGE gel electrophoresis and transferred electrophoretically onto a pure nitrocellulose blotting membrane (Pall Corporation, Pensacola, FL, USA).
