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All microbiological media were from Hi-Media (M/s Hi-Media, Mumbai, India).
Agar agar and yeast extract were from Becton, Dickinson and Co. (USA-France), peptone and MRS media were from Hi-Media (India).
Expansion medium contained MED-0001 and 5 ng/mL rhEGF, 5 μg/mL rhinsulin, 5 μg/mL transferrin, 10 nM T3, 1.0 μM epinephrine, 5 μg/mL hydrocortisone, 10% fetal bovine serum (all expansion media were from Wuhan Pricells Biotechnology & Medicine Co., Ltd).
HRP conjugated anti-mouse IgG and mounting media were from Vector Laboratories (Burlingame, CA) and Invitrogen respectively.
Lipofectamine 2000 and all cell culture media were from Invitrogen (Carlsbad, CA) and Alexa 488 and Alexa 546 were from Molecular Probes Inc. (Leiden, Netherlands).
All media were from Gibco (Invitrogen, Switzerland) and contained 2 mM L-glutamine, 100 U/ml penicillin and 100 µg/ml streptomycin.
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