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Subsequently, the media were filtered using a 0.22-μm pore filter (Steriflip, Millipore).
The media were filtered and analysed for silicic acid content.
After the incubation, cultured media were filtered, mycelial mass was harvested on sterile filter paper, and each sample was frozen in liquid nitrogen and then crushed.
Conditioned media were filtered with a 0.22µm-pore filter and stored in -80°C for further studies.
Virus-containing media were filtered through a 0.45 µm filter and applied to C2C12 myoblasts to generate C2C12-pMXIH vector control myoblasts and C2C12-FRG1 myoblasts which should stably express FRG1 protein.
Prior to use all media were filtered (0.22 μm).
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All culture media were filter-sterilized using a 0.2 µm filter.
Prior to use, all the media were filter-sterilized through a 0.22 μm filter (Millipore).
All media were filter-sterilized, instead of being autoclaved, to avoid changes in composition due to acetic acid evaporation.
Then, the media was filtered through a 0.2 μm pore strainer (Syringe filter), and then ultracentrifuged at 100,000 × g for 2 h at 4 °C.
Culture media was filtered with 100 µm size sieve to separate fungal pellets from media.
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