Sentence examples for media were discarded from inspiring English sources

Then, the cell media were discarded and replaced with fresh media.

After that, media were discarded and then replaced with 100 μl of fresh media.

All the media were discarded after 96 h by gentle pipetting.

For treatment purpose, old media were discarded and new culture medium (controls) or culture medium containing different concentrations of surface-coated SWSB was added to the wells for 24 h.

Media were discarded and cells were washed three times with PBS.

The media were discarded and fresh growth media were added without DIM followed by addition of 50 µl XTT [1 mg/ml in serum-free RPMI + phenazine methosulfate (25 nM ].

Then, the media was discarded and cells incubated with Lysotracker for two hours to allow the accumulation of the probe in lysosomes.

Afterwards, the old media was discarded and replaced with 100 μL of new complete media.

Upon 60% cell confluency, the spent media was discarded from the wells followed by washing with phosphate buffer saline (PBS) to remove unattached cells.

After predetermined time intervals, media was discarded from cel-seeded scaffolds followed by washing with PBS thrice and incubation with 200 μl of 5 mg ml−1 MTT solution (Sigma) at 37 °C for 4 h.

Then, 100 µL of media was discarded for all well-plates and compounds were serially diluted into the seeded cells at the concentration ranging between 30 0.47 µg/mL with cells treated with 3% DMSO (Sigma-Aldrich, USA) as the negative control.