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The porous media were conditioned to residual waterflood oil saturation prior to surfactant slug injection.
The efficacy of MSC-CM was found to be a function of the cell mass from which media were conditioned suggesting important pharmacological aspects of this treatment.
When conditioned media were used, an equivalent number of HEK293 cells or HEK293 cells expressing Wnt3A were seeded, media were conditioned for three to four days, collected, spun at 300g for 5 minutes and filtered through a 0.45 µM filter.
Media were conditioned with IL-1ra released from microspheres at intervals up to 20 days.
The normal growth media were conditioned by culturing BMSC/IFN- β or BMSC/vector for 24 h.
Media were conditioned with Nodal+/+ XEN cells (Nodal+/+ XEN CM), Nodal+/ − XEN cells (Nodal +/− XEN CM), or Nodal −/− XEN cells (Nodal −/− XEN CM) and incubated at 37°C, 5% CO2.
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To determine whether hMNP-CM had neurotrophic effects on neurite regeneration, media was conditioned for 48 hours in the presence of hMNPs and removed for presentation to axotomized cortical neurons.
To determine whether hMNP-CM had neurotrophic effects on neurite outgrowth, media was conditioned for 48 hours in the presence of hMNPs and removed for presentation to cortical neurons.
24 hr after transfection, serum-free media was conditioned for an addition 18 24 hr.
This media was conditioned for 24 h, followed by filtering and storing as described above.
Media was conditioned for 48 hours in the presence or absence of appropriate treatments.
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