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After confluence, the basic media were completed by 50 μg/mL of ascorbate-2-phosphate (Sigma).
All media were completed with 10% foetal calf serum (Invitrogen), no antibiotics were added.
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An explicit finite difference solution to the scalar wave equation in isotropic, inhomogeneous media is completed by filtering out non-physical contributions to the data.
Digitalisation of this media is completed on-site.
On day 2, once fragments had firmly adhered to the substrate, the volume of media was completed to 1 ml.
Culture media was complete 10% FCS RPMI-1640.
−His and −Ura media are complete media lacking histidine and uracil, respectively; -Trp-Leu is comedium medium lacking tryptophan and leucine (Rose et al. 1990).
Cell growth assays were completed in media with and without glycine as previously reported [27].
Cell treatments were completed using media lacking serum and containing either 1 μg/mL hydrocortisone (HC) (Sigma), 10 μM RU-486 (Sigma), or ethanol vehicle for 48 hours.
The voting -- by 126 members of the news media -- was completed after the final day of the regular season.
The most popular diplomas were media, which was completed by 884 students, and engineering, which was completed by 871 students.
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CEO of Professional Science Editing for Scientists @ prosciediting.com