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HPP-CFCs were first detected when two sources of conditioned media were combined to provide a synergistic combination of cytokines that supported the growth of primitive progenitors, a characteristic requirement of primitive progenitors.
The different release and physico-chemical properties possessed by the two starting polymers in various aqueous media were combined in the new matrix.
The moves, which drew laughter from the audience in the hall, and more than a little mockery on social media, were combined with some opening references to the disastrous time she had delivering her conference speech last year.
Harvested cells and exhausted media were combined and passed through a 70 µm cell strainer (BD Biosciences).
The semi-solid collection media were combined with the flush media and liquefied at 36±2°C for ∼15 minutes and the viral titers were determined by titration assay as detailed below.
The organic phases from the extracted culture media were combined and dried under vacuum.
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When serum-free, high glucose media was combined with mild (100 μU/mL) or moderate (250 μU/mL) concentrations of insulin, DMAQB-1 caused no statistically significant increase in DNA synthesis.
The Washington Post helps to illustrate the quantity of data that can be accumulated rapidly when social media is combined with traditional media surrounding a topic with a recent blog post focusing on Twitter volume during the 2012 Republican Presidential primary.
What made the campaign so powerful was that slick digital media was combined with what really matters – the hard work of organising an independent union of over 120 members in an effective and democratic way.
After 24 h, cells were pelleted and the conditioned media was combined with Laemmli sample buffer without reducing agent and run on a 7.5% SDS-polyacrylamide gel containing 1 mg ml−1 gelatin as previously described (Gum et al, 1997).
In Designing Media, Bill Moggridge and his interviewees show us that something more subtle is happening: pre- and post-digital media are combining in weird and complex ways neither one winning or losing, but each changing the other.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com