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The lentivirus-containing media were collected 48 h post-transfection and filtered to remove cells.
The conditioned media were collected and concentrated using a 3k MWCO concentrator (Amicon).
Cell media were collected 48 h after transfection.
Samples of all media were collected and stored at − 20 °C for analysis of silver concentrations.
The conditioned media were collected, and MMP-2 activity was determined by gelatin zymography.
Media were collected and protein concentration determined.
Conditioned media were collected after 4 hours for cytokine assays.
The next day, media were collected and cells lysed.
The spent media were collected and stored at −80°C.
Serum-free media were collected after 30 hours and filtered.
After 24 h of incubation, conditioned media were collected.
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