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Media were changed to fresh media containing A2E or control liposomes every 10 12 days.
Media were changed back to regular culture media for 5 days.
Media were changed after 24 h.
The media were changed every 3 days.
The culture media were changed 16 h after the electroporation.
Culture media were changed every 2 days.
Media were changed every 3 days.
The media were changed every 2 days once.
The media were changed to serum-free DMEM/F12 overnight before conducting the experiment.
The culture media were changed and the cells returned to the incubator.
The culture media were changed every alternate day (Kim et al. 2004).
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