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Ammonia yields in M9-YE medium and other nitrogen-containing media were calculated based on the nitrogen contents in the BD Bionutrients™ Technical Manual Third edition (BD Biosciences 2015).
Interaction energy profiles for CBNP porous media were calculated using DLVO theory for sphere plate geometry, demonstrating that the interaction energy for CBNP quartz sand was repulsive, whereas the interaction energies for CBNP IOCS and CBNP AOCS were attractive with no energy barriers.
The total cell numbers obtained from different media were calculated based on the cells cultured in the T-flask with DMEM+5% FBS (SC) medium reaching 90% confluency on day 3.
The statistical analyses for comparing the number of viable cells, background spots and detection rates in serum supplemented or serum-free media were calculated using a two sided, Student's t test for unpaired samples (P < 0.05).
The uptake rates for the simulated media were calculated as follows: (1) carbohydrate content was defined as 50% mono- and disaccharides and 50% polysaccharides; fat content was defined as equal fractions of glycerol, cholesterol, saturated fatty acids, and unsaturated fatty acids; and protein was assumed to consist of equal fraction for all 20 amino acids.
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The dynamical viscosity in such media is calculated.
Effective thermal conductivity for porous media was calculated by means of the finite volume method.
The effective thermal conductivity of nanofluid in porous media is calculated using copper powder as porous media.
The work of adhesion (WA) by the probing media was calculated by use of advancing contact angle data.
The effective bulk and transport parameters for isotropic porous media are calculated solving the corresponding closure problems.
The recoil surface areas of the porous media are calculated through the fractal quantification of the synthetic porous media.
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