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Because K2HPO4 is the only source of K+ in Chu growth media, we added a constant amount of KCl to all test tubes to insure that K+ did not become limiting as K2HPO4 decreased along the N∶P gradient.
In all media we added 51 mM NaCl as in the classical preparation of NGM [ 71], except in the salt environment where the concentration was 308 mM NaCl.
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For differentiation, cells were plated on Matrigel and bFGF was not added to the growth media; instead, we added 20 ng/ml NT3 and 20 ng/ml BDNF to sustain the differentiated neurons.
In a world of social media, we've added Twitter followers and Facebook fans to the mix.
Entertainment hubs Los Angeles and New York City are over 50% female and over 50% people of color, and yet over 70% of episodic and theatrical actors are White, and as Geena Davis relayed to NPR in 2013 - citing the research of her Institute on Gender in Media - "If we added female characters at the rate we have been over the past 20 years, we will achieve parity in 700 years".
In the world of interactive digital media, we might add two categories.
"This started with helping people with their own personal media libraries, and then we added over-the-air television, news, podcasts, and now web shows.
With the popularization of Facebook, Twitter, Instagram and more, we added Social Media as a channel, we created infrastructure, roles, strategies, to deal with the precise new skills we needed to perfect media in an age of sharing.
The next day, we added growth media with 1% 4× charcoal-stripped serum with or without the different xenoestrogens or vehicle.
We added the media-only wells to Protocol 2 and for all protocols analyzing proliferation assays the subtraction of media-only wells from the wells with cells is indicated for each GFP fluorescence reading.
When using Phenol-red free media we used to add Sodium pyruvate from Cellgro (Cat #25-000-CI) to a final of 1 mM.
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