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For experiments where the media was sampled, the last media change employed DMEM without phenol red.
Cell culture media was sampled to determine OD600nm and substrate consumption.
The media was sampled from the abluminal compartment (50 µl) and replaced with the equal volumes of fresh media to maintain appropriate sink conditions.
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Different environmental media were sampled.
Culture media were sampled, and levels of TNF-α were measured by ELISA (R&D systems).
Although the authors state which media were sampled for the study, detailed field documentation in Jayatilake et al. is not provided.
Driscoll said that familiarizing people with even small pieces of opera music -- "whether it's [through] YouTube, whether it's social media, whether it's sampling an aria" -- will get people interested in opera.
Remix artists have been sampling media broadcasts since at least 1956, when Bill Buchanan and Dickie Goodman decided to mash up a bunch of contemporary hits with a radio report of an alien invasion for "The Flying Saucer".
One milliliter of conditioned media was removed for sample analysis.
For the SKC sampler, the UTM collection media was removed from the sampler and placed a storage tube.
1.5 ml of the original sample media was spiked with 10 µl internal standard (IS, ribitol, 2 mg/ml dissolved in water) and freeze dried.
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