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Media was removed and replaced with fresh media with or without 10 ng/ml TGFβ1.
Media was removed each well and replaced with fresh media containing 1 mg/mL MTT.
Neutrophil media was removed and cells washed with fresh RPMI.
After 24 h, culture media was removed from wells.
Afterwards, media was removed and washed twice with PBS.
The old media was removed and wells containing adherent cells were washed with PBS.
The media was removed, and cells were washed twice with PBS.
At the end of the experiment, the media was removed and washed twice with PBS.
Twenty-four hours after transfection, culture media was removed and replaced with phenol-red free media.
After 24 hours, media was removed and 100 µL/well fresh medium containing protein was added.
One hour later, the media was removed and replaced with B-27 and Neurobasal medium.
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