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The incubation period of 48 hours in the serum-free media was optimized to maximize secreted protein concentration while minimizing cell death.
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Four important components of growth media were optimized by central composite design.
Their bifunctional activities for electrochemical energy storage and electro-catalysis for water splitting in strong alkaline media are optimized by varying the ratio of concentrations of the Ni and Co precursors.
A commonly used cyanobacterial growth media (MLA) was optimized to maximize biomass production.
In an attempt to increase its anti-Agrobacterium tumefaciens activity, GYM + S (glucose, yeast extract, malt extract and starch) medium was selected out of five different production media and the medium composition was optimized.
Specifically, eGFP expression was successfully optimized for high expression in rich media, and the CatA enzyme was optimized for high expression in stationary phase growth, with the highest expression variant resulting in 2.9-fold higher product yield over a commercially optimized variant.
HPLC analysis showed galactose at the retention time of 2.29.The maximum EPS yield was optimized for the media components like glucose (20 g/L), yeast extract (25 g/L) and ammonium sulphate (2 g/L) using Central Composite Design and Response Surface Methodology (RS M.
We evaluated these three datasets for M. tb and compared them to gene deletion analyses for iNJ661, in which each gene was individually deleted and growth was optimized on Middlebrook media.
First, growth of hairy roots was optimized for different solid media and it was found that WPM supported better growth.
The culture media for glycerol to ethanol biotransformation by Escherichia coli under anaerobic conditions was optimized.
The gap placement was optimized iteratively.
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