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Culture media was filtered with 100 µm size sieve to separate fungal pellets from media.
Culture media was filtered through cheese cloth to separate the mycelia from the broth.
The fermentation media was filtered and filtrate was extracted thrice with DCM, dried over anhydrous Na2SO4, and evaporated in vacuo.
For each infection, the collected media was filtered through a 0.45 µm filter to remove cell debris and then equal amounts of fresh RPMI supplemented with 10% FBS was mixed with the filtered media with 8 µl of polybrene (1 mg/L) per 10 ml medium.
The insect culture media was filtered before purification.
Two days after transfection, the cell culture media was filtered using a 0.45- μm syringe filter.
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LB media was filter sterilized and cultured with the OP50, HT115 or specified RNAi strain used to seed NGM plates.
The media were filtered and analysed for silicic acid content.
After the incubation, cultured media were filtered, mycelial mass was harvested on sterile filter paper, and each sample was frozen in liquid nitrogen and then crushed.
When our media is filtered and refiltered, bleached by content guidelines and automatic takedown algorithms, we run the risk of living a life with the objectionable and graphic and terrifying, in other words the real, strained out.
Conditioned media were filtered with a 0.22µm-pore filter and stored in -80°C for further studies.
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