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The cells were cultivated in selective media in the first stage, and then transferred continuously to the second stage where the media was enriched by feeding yeast extract.
Fed-batch cultures were grown in 5 cm diameter Drechsel's bottles with a 250-ml working volume and bubbled using air enriched with 5%% CO2 (v/v); in this case, F/2 growth media was enriched with added nitrogen, phosphate and iron sources (0.75 g/L NaNO3, 0.05 g/L NaH2PO4 and 0.0063 g/L FeCl3·6 H2O final concentrations).
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For cell culture experiments, the media were enriched to 5%D200 thus p is 0.05.
Middlebrook 7H10 media were enriched with 10% sheep blood hemolyzed by 1 1 dilution with water and subsequent freezing thawing.
Proteins in the media were enriched by precipitation in 50%% methanol at -20 °C, pelleted by centrifugation and dissolved in SDS-PAGE sample buffer.
Isotopic labelling for NMR studies was achieved by culturing the E. coli strain in M9 minimal media, which was enriched with either N ammonium sulfate or C glucose and N ammonium sulfate (Goss Scientific).
The evening was enriched by reminiscences.
My life was enriched by her".
New York was enriched by slavery, and then enriched again by the war effort.
Transfection was enriched by hygromycin selection.
Before sanctions, Iran was enriching below 5percentt.
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