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The powdered media was dissolved in 900 ml of Millipore water in an autoclaved glass conical flask under sterile conditions.
The quantity of 0.3 g/30 mL of RPMI 1640 media was dissolved in (10 g/1000 mL) distilled water and added to 20 separate small screwed caped tubes.
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Set in a not-very-far future, where American power is collapsing and ubiquitous social media are dissolving both print culture and any sense of privacy, it's unsettlingly plausible.
But the growing role and power of tech platforms as media distributors suggests the communal consensus represented by the notion of 'mass media' is dissolving precisely because algorithmic priorities are so singleminded in their pursuit of engaged eyeballs.
After 4 days of transfer to fresh media, the substrate 1 (600 mg) was dissolved in DMSO (10 mL) and evenly distributed among 10 culture flasks (1 liter) having 400 mL cell suspension media under aseptic conditions, which were kept for 15 days under the same conditions.
PDTC (Sigma-Aldrich, St . Louis MO) was dissolved in media and filter-sterilized.
N-acetyl-l-cysteine (Sigma) was dissolved in media containing HEPES (4- 2-hydroxyethyl -1-piperazineethanesulfonic acid) buffer, adjusting pH using 1 ℳ NaOH.
After removing the culture media, the purple formazan crystal was dissolved in 200 μL of DMSO plus 25 μL of Sorenson buffer as reported.
25-hydroxy (25-OH) cholesterol (Sigma) was dissolved added to media as indicated in figure legends.
The middle layer was collected, centrifuged at 500 g for 10 min, supernatant was removed and pellet was dissolved in growth media.
Firstly, it was administered exogenously wherein α-KG was dissolved in the media to get a final concentration of 2 mM, and 1 mL was added to each well (2-D and 3-D controls were wells without α-KG).
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CEO of Professional Science Editing for Scientists @ prosciediting.com