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Members of parliament have complained that they have been kept in the dark about the attack that - according to German media - was detected in December.
Accumulation of hBD-2 and LL-37 in the culture media was detected by slot blot [40].
When testing the group I protein AtBPC1 for homo- and heterodimerization, a weak growth on selective media was detected accompanied by β-galactosidase activity (Figure 3B).
SDF-1 in the culture media was detected by an enzyme-linked immunosorbent assay (ELISA) kit from R&D Systems.
Endogenously active MMP-1 activity in culture media was detected using a SensoLyte Plus MMP-1 assay kit from AnaSpec.
The absorbency of samples of these replaced media was detected by an UV spectrophotometer at 326 nm.
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Accumulated amounts of lactate in culture media were detected using the lactate assay kit.
Interestingly, 2-hydroxyisobutyric acid and 2-hydroxyisovaleric acid, neither of which are included in published recipes of TeSR-1 media, were detected by 1H-NMR in UM.
Cytokines and matrix metalloproteinases released into the media were detected using antibody arrays and quantified by ELISA.
Only ten cases of bacteremic otitis media were detected and of these, one case was caused by serotype 3. The patient survived.
The protein expression of MMP-2, MMP-9, E-cadherin, N-cadherin, vimentin, and actin in McA-SYYCM and McA-nSYYCM conditioned media were detected by western blot assays as previously described with slight modifications [ 28].
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