Sentence examples for media was decanted from inspiring English sources

Media was decanted and the pellet resuspended in the alternative media.

Blocking R10 media was decanted and 100 µL of peptide (1.5 µg/mL final concentration), PHA or Mock were added followed by 50 µL of cells to give a density of 200,000 cells/well.

After 24 hrs, the growth media was decanted to remove non-adherent cells and then replaced with fresh R10+ media.

YM media was decanted and the yeast cells reconstituted to 500 mL using yeast nitrogen base (Formedium, Hunstanton, UK).

After this time period, the media was decanted (with 0.5 mL reserved for HPLC analysis), and the plates were rinsed twice in PBS.

The following morning (144 hr), the cell media was decanted and replaced with DMEM low-glucose media (glucose 2.5 mM) for 5 hr.

Extracellular media were decanted and cells were washed three times with 1 mL phosphate buffered saline (PBS).

The culture media were decanted, the cells washed with PBS (3 × ) and then incubated with 4 ml fibroblast CM for 48 h.

Briefly, media were decanted and monolayers fixed with 10 % TCA, washed with tap water, dried, stained with 0.4 % SRB in 1 % acetic acid, de-stained with 1 % acetic acid, dried, solubilized with 10 mM Tris base and quantitated by spectrophotometric detection at 490 nm using a plate reader.

Starter cultures were prepared by growing cells in 20 mL of LB media and then spinning the cells at 4800 g for 5 min. The LB supernatant was decanted and the cell pellet resuspended with 20 mL of minimal media to inoculate 1 L of minimal media.

The cell culture media containing different concentrations of tested extracts and dead cells was decanted, and viable attached cells into the tissue culture plate were leaved.