The concentration dependence of the absorbance for the solutions in the different media was collected from three different solution preparations.
Media was collected, TCA-precipitated and subject to immunoblot analysis.
The media was collected 6 hours later for the α-MSH RIA.
Media was collected at 8, 16 and 24 hours, filter-sterilized and stored at −80°C.
Two days after infection cell culture media was collected and analyzed for GLuc concentration.
Three days post-infection, media was collected and centrifuged at 2500RPM to remove any cells.
The MEM media was changed twice a week and spent media was collected to accumulate spores.
After 18 h, media was collected, (TCA) precipitated and analyzed by immunoblotting procedures under reducing conditions.
Virus containing cell media was collected after 48 hours of infection.
The media was collected and exosomes isolated by centrifugation at 100,000 g for 1 hr.
Conditioned media was collected from DF-1 RCAS-HRG cells after 3-5 days incubation.
