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Last year, Gizmodo shot to international notoriety after it obtained a prototype of Apple's hotly anticipated iPhone 4. Gawker Media was cleared of all wrongdoing by California police earlier this month.
The media was cleared of cells and debris by centrifugation at 16,000 × g for 2 minutes.
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Iodinated agents that are typically used in the clinical setting cannot be used for intravenous injections in rodents because microCT devices are not fast enough to image animals before the contrast media is cleared from the blood.
For assay of ePPi, media were cleared of cellular debris and were diluted 1 2 in lysis buffer.
Infected cell culture media were cleared of cell debris by low-speed centrifugation and collected as stocks of cell-free virus.
The collected media were cleared by centrifugation (10 min, 1000× g, 4°C), adjusted to pH 8.5, filtered through a 0.22 μm PVDF membrane and applied to a 1 ml Protein A Hitrap column (GE Healthcare).
In the treatment group the media was clear in 50% of eyes in the immediate postoperative period compared with only 8% in the control group.
There were 58 counts of sexual assault that night, and even though only three arrested suspects were recent refugees, the subsequent message from Europe's right-wing media was clear: Refugees are a threat to the safety of women in the EU.
Both eyes had visual acuity of 20/32 or better measured by a standard protocol, ocular media was clear enough for good quality fundus photographs, and there was absence of any ocular disorder that might obscure assessment of either AMD or lens opacities.
As long as the refractive media was clear enough for fundus examination after surgery, we confirmed whether the patients met recruit criteria.
Of these, 13 high myopic eyes and eight control eyes were excluded because the border between the choroid and the sclera could not be visualized, although the optical media was clear.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com