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At 4th day adipocyte differentiation media was added by subsequent changes in every 2 days.
The chamber slide was incubated at 37 °C overnight for attachment and fresh media was added.
Then, 900 μl of the culture media was added to the wells.
Fresh media was added on the fifth day.
Fresh media was added after every 2 days.
Media was added to one row as a negative control.
In each well, conditioned media was added to the cell containing solution to bring the volume to 75 µL, to which 75 µL fresh media was added.
Then, fresh media was added and the media changed the next day.
Prior to the experiment, old media was aspirated off and 150 µL fresh media was added.
After 48 hours infection, fresh media was added containing 2 µg/ml puromycin.
Fresh media was added every second day and cells were grown to approximately 60% confluency.
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