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After this period, the media volume was suctioned and replaced with media containing 10%% FBS for 24 h.
After that, the media volume was suctioned and replaced with DMEM media containing 10% FBS for 24 h.
The basolateral media volume was significantly larger than the apical volume (10× or more) which would have largely negated the effects of basolateral accumulation of DTPA or mannitol.
A volume of MTT solution equal to 10% of the culture media volume was added to the cell culture at 37°C for 3 hrs.
A solubilization solution (10% Triton X-100 and 0.1 N HCl in anhydrous isopropanol) in a volume equal to the culture media volume was added and further incubated at 37°C until the resulting formazan crystals were completely dissolved.
Half of the media volume was changed every day.
Similar(49)
An extensive matrix of different growth conditions including media, incubation time, inoculum volume, surface area and media volume were investigated in order to maximize the yield of bacterial cellulose produced by Gluconacetobacter xylinus, which will be used as reinforcement material to produce fully biodegradable composites.
Platelets were added to the culture media at a concentration of 100,000/μL (total culture media volume is 2 mL).
However, if the cell number and media volume are not considered, this will likely result in applying different doses of ligand per cell across the different experiments, which may lead to experimental inconsistencies.
The cells underwent media changes every 48 h but to avoid disturbing the cells only 80% of the media volumes were changed.
While, media maintenance volume was 20 L.
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