The tissue compartments and the interconnecting microfluidic channel contained a total media volume of 610 µl.
Medium was subsequently replaced with WEM supplemented with Cell Maintenance Supplement Pack (Invitrogen) and 50% of the media volume was replaced every 48 h.
The effect of elevated organic carbon concentrations on total ammoniacal nitrogen (TAN) removal rates was evaluated and determined based on biofilter media volume.
The small media volume per cell and the development of shallow concentration gradients allow factors secreted by the cells to rise to bioactive levels.
Moreover, the operation times of AMBR and AMBRb were 4.2 and 3.5 times longer, respectively, than that of SMBR at an optimum media volume fraction of 30%.
Changes in device material, surface coating, cell number per unit surface area or per unit media volume may all affect the outcome of otherwise standard protocols.
Differences were even observed when using fairly similar and simple incubations setups with mere changes of the media volume, under maintenance of sink conditions, and of the vessel geometry.
Three sets of submerged membrane bioreactors (MBRs), including one suspended-growth MBR, called SMBR, and two attached-growth MBRs (one called AMBR, and another with baffles called AMBRb) were operated at various media volume fractions.
As Time magazine later reported, a P.R. outfit called Creative Response Concepts immediately pumped up the media volume of her supposed martyrdom, breathlessly producing a former Alito clerk to provide eyewitness testimony of her suffering at the hands of those Democratic brutes.
Mannitol (150 and 300 mM) was added to the basolateral media (volume 300 μL).
Lack of dissolution information (e.g., missing information on dissolution media volume, individual dissolution data, multimedia dissolution, etc).
