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Nuclei were counterstained with DAPI in Vectashield mounting media (Vector Labs).
Slides were mounted with mounting media (Vector Labs, USA).
The slides were mounted with vectashield mounting media (Vector) and viewed under an Olympus Fluoview FV1000 confocal fluorescence microscope.
After a final wash, the sections were mounted, taken through Sudan Black to reduce autofluorescence, and coverslipped with Vectashield mounting media (Vector).
The slides were mounted with Vectashield DAPI (4'-6-Diamidino-2 phenylindole) mounting media (Vector H1200, USA) to counter-stain the nucleus, nail-polish sealed and kept in dark at RT for immediate microscopy.
Unbound secondary antibodies were removed by washing in PBS and sections were mounted under glass cover slips using DAPI mounting media (Vector Labs, U.K .. Negative controls were performed by replacing the primary antibody with PBS.
Similar(44)
Strikingly, 5-FU-mediated cell death was abrogated by pretreatment with SPINK1-CM but not by control media (vector-CM), however to a degree lesser than that observed in MCF-7 SPINK1OE cells (Fig S7 of Supporting information).
Coverslips were mounted on slides with vector shield mounting media (Vector Laboratories).
Coverslips were rinsed three times in washing buffer and then mounted onto glass microscope slides with 5 μl of Vectashield mounting media (Vector Labs Ltd ,UK) and sealed with nail varnish.
Sections were washed three times with PBS before mounting in DAPI-containing mounting media (Vector Laboratories, Inc., Burlingame, CA).
Sections were then stained with DAPI (1∶1000, Invitrogen Ltd ,Carlsbad, CA, USA) for nuclear staining and mounted with Vecta shield mounting media (Vector Laboratories, Burlingame, CA).
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