[32] Prior to each experiment, the bacteria, cultured aerobically in LB media, were diluted into fresh LB media and cultured under continuous 200 rpm shaking at room temperature to a concentration of 1.8 × 108 cells mL-1.
Staff were said to be severely disciplined for innocuous social media activity, worked under continuous surveillance, and could be reassigned from office work to cleaning and factory jobs if they were deemed noncompliant or useless.
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Col wild-type and sis7-1/nced3-4/sto1-4 seeds were sonn on minimal media for 20 h and transferred to 100 mM Glc or equimolar sorbitol (as an osmotic control) media for 13 h under continuous light to allow induction or repression of Glc-responsive genes.
A mono-lung cancer cell line, a mixture of lung cancer and stromal cell lines, and cells from fresh lung cancer tissues were cultured in 3D under continuous media supplementation, mimicking the actual tumor microenvironment in vivo.
Four to five surface-sterilized seeds of Arabidopsis thaliana Columbia (Col-0) were sowed on a 3 cm-diameter cellophane disc of type 325P (AA packaging Ltd ,Preston, UK), placed on growth media and incubated horizontally under continuous light for 4 to 5 days.
For Glc regulation of ABA biosynthesis gene expression, seeds of Col, sis7-1 and sis7-3 were stratified and sown on nytex screens on minimal media and incubated for 20 h under continuous light and then transferred to 300 mM Glc or sorbitol media for an additional 12 h under continuous light before harvesting.
Arabidopsis thaliana (Col-0) seedlings were grown in 0.5 × MS (Casson, North Logan, UT) media in a Percival chamber at 22°C under continuous illumination (90 μmol m-2 sec-1).
The impact of different carbon sources on biomass growth was studied in shake flask experiments under continuous illumination using BG-11 media and 5 mM of either carbonate, acetate, glucose or glycerol.
