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Media transferred from sham-exposed cultures and media irradiated in the absence of cells were used as controls.
Gene expression analysis of fibroblasts incubated for 24 h with Claycomb media transferred from cardiomyocytes, resulted in 333 differentially expressed genes compared to fibroblasts incubated with unused Claycomb media (Table 2) (Table S1).
Cells are harvested when they began to round (16 17 hr p.i). by scraping together into the media, transferred to a 50 ml sterile tube and freeze-thawed 3 times by passing the tube from dry ice-ethanol (−78°C) to 37°C water bath.
Trypsinised cells were then divided equally into an appropriate number of tubes depending on the number of media being trialled and pelleted by centrifugation at 800 x g for 5 min. The cell pellets were resuspended into appropriate media, transferred into tissue culture flasks (Corning) and incubated in a humidified incubator with 5% CO2 at 37°C.
In addition, cells cultured in media transferred from hypoxic HMEC-1 cells took on an altered morphology with more rounding up of cells.
Some sampling cartridges can be sealed and transported and the sampling media transferred to Soxhlet or pressurized liquid extraction systems in a clean laboratory environment.
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Backfilling behind the supports makes the steel supports fully contact with rock surface, increases the stiffness of media transferring resistance forces from steel supports to surrounding rocks, and greatly improves the performance of the steel supports.
Seeds were germinated on sterile media and transferred to media with and without 50 µM of 5-azaC 3-days-post germination (dpg).
Likewise, spheroids formed by budding in serum-containing media were transferred to spheroid media prior to drug treatments.
Thereafter, the media was transferred to osteoinductive media with 10−8 M dexamethasone for a further 7 days.
The reciprocal result was observed when cells grown in CR media were transferred into expired NR media.
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