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For media transfer experiments, cultures grown in the presence of fructose (control) were inoculated with 10 wild-type A. nidulans conidia were inoculated in minimal medium supplemented with 25 mM fructose for 16 h at 37°C, 150 rpm.
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For the transfer experiments, coverslips were washed three times with water and then transferred to minimal media supplemented with a different carbon source for 5 h.
Transduction and DNA transfer experiments.
Optical media transfer and electronic submission.
§ 225.37 Optical media transfer and electronic submission.
For serial transfer experiments, CFSE-labelled CD4+ T cells were transferred into mice for the primary transfer.
49 CFR § 225.37 - Optical media transfer and electronic submission.
Reciprocal transfer experiments were performed as follows.
The nuclear transfer experiments support this view.
More recent mitochondrial transfer experiments support the general findings of the nuclear transfer experiments (50, 51).
For conditioned media experiments, media was collected from non-depleted cells each day and transferred to depleted cells after each media change.
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