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Thus, P4 ASC were incubated with specific culture media to induce both osteogenic and adipogenic differentiations in comparison with un-induced controls.
It was concluded that a constructed sub-surface flow wetland design should incorporate a combination of higher aspect ratios, higher loading rate and finer porous media to induce a hydraulic behaviour closer to an ideal plug flow system.
1 ng/ml EGF was added to the media to induce migration.
One day after transfer, EGF was omitted from the media to induce differentiation.
At time 0, low calcium media was replaced with normal Ca++ (1.8 mM) media to induce cell-cell adhesion.
Third, hBili siRNA synergized with WNT3A conditioned media to induce transcriptional upregulation of the endogenous β-catenin target gene, AXIN2 (Fig. S5F).
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Further, our experiments with cells precultured on nitrogen starved media failed to induce the expression of FLO11 when transferred to media containing inducing concentration of ammonium sulphate.
At this point IBMX (500 μM), dexamethasone (2 mM) and indomethacin (125 μM) were added to the media overnight to induce differentiation, before changing back to the growth media with just T3 and insulin added which was changed every 48 hr.
Since November, India has employed a diplomatic and media campaign to induce the international community — the United States, in particular — to put greater pressure on the Pakistan Army to break its ties with jihadi groups.
The omission of media supplements to induce osteogenic differentiation is a promising feature towards simplified and cost-effective cell culturing procedures of a potential bioengineered product, and concomitant translation into the clinical field.
For the detection of differentiated derivatives, hES cell-derived EBs were plated on gelatin-coated 12-well plates to allow them to adhere, grown in DMEM (Invitrogen) containing 10%FBS (HyClone, Logan, UT) or Primary Neuron Growth Media (Lonza) to induce further differentiation for 14 days, and fixed in 4% paraformaldehyde followed by immunocytochemical analyses.
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