Sentence examples for media to exponential from inspiring English sources

Yeast strains CMY288-1B, CMY8058-3-4 and CMY8082-20-3 were grown in YPAD rich media to exponential mid-log phase (OD600 = 1.0, 500 mL culture).

In all complementation analyses, cells were pre-cultured on LB media to exponential growth phase, harvested by centrifugation, and washed for three times with M9 minimal media without any carbon sources.

A 1 ml frozen aliquot of transformation stocks was thawed out and grown in competence media to exponential phase (OD600nm = 0.4) at 37°C in a 5% CO2 incubator.

All species were initially cultivated in species-specific media to enable exponential growth (Table S3).

Strains were grown in M9 minimal media to early exponential phase.

The transformed cells, and untransformed negative controls were grown in rich media to mid-exponential phase with aeration.

Cultures were grown in selective media to mid-exponential phase before treatment with 200 mM CaCl2 for 10 min. Cultures were then harvested and whole cell extracts were prepared from each sample, resolved by Phos-tag SDS-PAGE, and immunoblotted as above.

Cells grown at 30°C and 25°C on methanol media to the mid-exponential growth phase were shifted to 0.5% glucose in order to repress the P AOX and aliquots of equal volume were taken at regular time intervals after the shift.

Prior to infection overnight cultures of bacterial strains were diluted in new media and grown to exponential phase (OD 0.8 to 1), washed three times in serum-free mammalian cell culture media and resuspended in mammalian cell culture media at the indicated MOI.

Colonies were picked into SD-LU media and grown to exponential phase at 30°C.

Cells were pre-cultured on rich chocolate agar plates to confluence and minimal Chamberlain media with glucose to exponential, mid-log, and stationary phase.