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The MCF-7 cell line, which originated from human breast cancer mammary epithelium, was cultured in regular growth media that consisted of RPMI-1640, 10 % FBS and 1 % penicillin/streptomycin.
The 4T1 cell line, which originated from a murine mammary adenocarcinoma, was maintained in regular growth media that consisted of low glucose DMEM, 10 % FBS and 1 % penicillin/streptomycin.
Mouse C3H10T-1/2 MSCs were acquired from ATCC and were maintained using growth media that consisted of Dulbecco's Modified Eagle Medium (DMEM) that was supplemented with 1% penicillin-streptomycin antibiotics (100 U⋅mL−1) and 10% fetal bovine serum (FBS; HyClone Logan).
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We simulate a turbid media that consists of three layers with refraction occurring at their interfaces.
We simulate a turbid media that consists of multiple layers, shown schematically in Fig. 1.
Feeder-free and xenobiotic-free defined culture media that consist of a combination of recombinant growth factors known to inhibit differentiation and maintain hESCs in the pluripotent state are now commercially available.
Reaction media that consist of organic solvents can greatly expand the lipase-catalysed transformation repertoire, where numerous reactions might be used by synthetic and polymer chemists with high selectivity [ 34].
We simulate a turbid media that consists of three layers, shown schematically in Fig. 2. The first layer extends from 0 µm to 330 µm, and the third layer extends from 360 µm to 1.2 mm, have an absorption coefficient µ a = 1.5 cm−1 and a scattering coefficient µ s = 60 cm−1.
Another single-center study that systematically tested the impact of different freezing media on cell viability and T-cell function compared four different media additives that consisted of (1) fetal bovine serum, (2) autologous plasma and Dextran-40, (3) human AB serum, or (4) human serum albumin [ 18].
For primary sphere formation, cells were plated in nontreated, low adhesion, 96 wells plate at the concentration of 100 cells/100 μL/well in stem cell media (SCM) that consisted of DMEM F12 AA (Gibco), supplemented with 1× B27 (Gibco), 20 ng/mL epidermal growth factor, and 10 ng/mL fibroblast growth factor (Sigma).
For his Totentanz-project in Basel, Peter Greenaway conceived a multi-media installation that consists of 18 funerary monuments, which serve as displays for films that depict various ways to come to death.
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