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Vero cells were grown as monolayer in media supplemented with 10% inactivated fetal bovine serum.
In media supplemented with lactose, cells were allowed to grow for 24 hours.
The media supplemented with 6% of HW and FW enhanced keratinase production.
Fed-batch fermentation was initiated with 2TY media supplemented with 0.1 M glucose.
HEK293 and CHO cells (from ATTC) were grown in appropriate culture media supplemented with 10% FBS.
Blood was added to 5 ml complete RPMI1640 media supplemented with 0.3 ml heparin.
Similarly SAECs were maintained in SABM media supplemented with growth factors.
Diploid yeast were grown on SD media supplemented with histidine and uracil (SD2) for 3d.
Media supplemented with methanol alone were used for plates without rapamycin.
The HAEC were cultured in media supplemented with 5% FBS as per the manufacturer's instructions.
hMSC were grown in MEM alpha media supplemented with antibiotic-antimycotic and 10% premium select FBS.
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