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However, further improvement is needed because even the most extensively engineered baculovirus/insect cell systems require media supplementation with N-acetylmannosamine, an expensive sialic acid precursor, for efficient recombinant glycoprotein sialylation.
The present study utilized a 2 × 2 × 2 factorial design examining age (old vs. young), follicle size (≥2 mm vs. <2 mm) and media supplementation (with or without fetal bovine serum [FBS]) to determine factors that might affect in vitro maturation of alpaca oocytes.
Interestingly, media supplementation with the antioxidant glutathione largely restored fly tolerance to oxidative stress.
A common approach to improve heme supply is media supplementation with δ-aminolevulinic acid, a precursor molecule of the heme biosynthesis pathway (e.g. Ryan and O'Fágáin 2008).
Several groups have demonstrated in vitro differentiation of PGCs from both mouse ESCs and hESCs via media supplementation with bone morphogenetic proteins (BMPs) (3, 5, 7, 19).
Serum-free culture media supplementation with EDPs (Kel 50 μg ml−1) for 6 48 h did not induce HT-1080 cell growth.
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Other culture media supplementation showed no significant differences between droplets, with or without zygotes/embryos.
Calcium free DMEM media with supplementation with 5% FBS plus calcium (final concentration 0.6 mM) proved optimal from a variety of media compositions tested for lamellar propagation to passage 4 on collagen type I substrates (Table 1) resulting in homogenous populations of small cobblestone shaped proliferative cells.
As noted by Primm et al [ 20], M. tb growth on Middlebrook 7H9 media requires supplementation with glucose and glycerol.
Heterologous expression of BnBBM also successfully induced SE in N. tabacum, although the media required supplementation with cytokinin to achieve regeneration [ 14].
It is worth noting that experiments using media supplemented with yeast extract (in Table 1B), were performed at higher dilution rate, because this supplementation allowed the increase from 0.08 to 0.12 h−1 without losing stability in the continuous cultures.
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