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A heterogeneous sample comprising a mixture of cells and cell media solution was loaded into a 1 ml syringe reservoir and each droplet was printed at pre-determined positions on a glass surface or into a 96-well plate.
CLONfectin/DNA- media solution was removed and then cells were washed with medium or 1× phosphate buffer saline (PBS).
Cells to be transfected were removed from the cultures and the CLONfectin/DNA media solution was gently applied.
The bottom agar layer contained 1.5 ml of 0.6% agar solution and 1.5 ml of media solution was poured and plated.
A 7.5% (wt/v) gelatin (Sigma) in media solution was made, which forms a solid at room temperature but melts at 37 °C.
The GzmB/inhibitor media solution was transferred to wells that had been previously coated with FN and blocked as described above, before seeding of cells.
Similar(54)
Cells (4 × 104) in 4 ml of a 0.35% agar-MCF10A media solution were plated in triplicate on 35-mm-diameter dishes containing a 0.7% agar plug (BiTek, DIFCO Laboratories, Detroit, MI, USA).
The Casiopeína III-ia and the different aqueous media solutions were degassed before being loaded into the syringe and the reaction cell of the calorimeter.
All reagents, protein, and media solutions were prepared using water that had been passed through a Milli-Q ultrapurification system (Merck Millipore , Inc. to achieve a resistivity of 18 MΩ.
Because of the instability of STZ in aqueous media, the solution was made using cold citrate buffer (pH 4.5) immediately before administration.
Hibernate-A (1 mL) was again added back to the vial and the media-bead solution was pipetted vigorously to resuspend beads in media.
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