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Exact(7)

The modern methods of bacteriological technique had their beginnings in 1870 85 with the introduction of the use of stains and by the discovery of the method of separating mixtures of organisms on plates of nutrient media solidified with gelatin or agar.

Thus far it has been impossible to render these strains axenic; however, the total number of bacterial strains was successfully reduced by lysozyme treatment (following [26]) and pour plating in low-phosphate MLA media solidified with agarose.

Stock BY-2 calli were maintained on media solidified with 0.7% (w/v) agar and subcultured monthly.

The epithelia were then oriented in the plane formed between two successively formed blocks of the same media solidified with 9% glutaraldehyde.

Although cotyledons formed callus on all hormone combinations in media solidified with Microagar, no shoot regeneration occurred after seven weeks of culture with one exception, while the explants on Phytoblend-solidified media did show shoot regeneration.

Callus cultures of wild-type N. benthamiana were generated by placing leaf and petiole tissue explants from aseptically-grown seedlings onto MSG media solidified with 6 g/L agar.

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Similar(53)

For growth on plates, media were solidified with 15 g.l−1 Bacto-agar (DIFCO Laboratories, Detroit, USA).

The media were solidified with 2% (w/v) bacto agar.

Growth media were solidified with 15 g/liter agar (Difco) for the preparation of plates.

Media were solidified with 20 g/l agar (ForMediumTM) and strains were incubated at 28 or 37 °C.

Growth media were solidified with 15 g/L agar (BD Diagnostic System) for the preparation of plates.

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