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Media components – Bacto – agar, Yeast extract, Tryptone, Tissue – culture media; sodium bicarbonate; trypsin – Sigma Chemicals Co., USA.
A variety of fatty acids were tested in the growth media: sodium butyrate, pentanoic, hexanoic, heptanoic, sodium octanoate, decanoic, and dodecanoic.
Furthermore, the use of micellar media (sodium dodecyl sulphate, SDS) as a carrier and the addition of tetrahydrofurane (THF) in the PO solutions increase both the solubility and stability of POs, avoiding their rapid degradation in water.
Then cells were washed and 590 μL of non-buffered media (sodium bicarbonate free, pH 7.4 DMEM) was added to each well.
Cell culture media, sodium dodecyl sulphate (SDS)–polyacrylamide gel, polyvinylidene difluoride (PVDF) membrane, and bovine serum albumin (BSA) were purchased from Invitrogen (Carlsbad, CA, USA).
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Cell culture medium [MEM and DMEM/F-12 (1:1)], fetal bovine serum (FBS), MEM vitamin solution, non-essential amino acids, epidermal growth factor (EGF), insulin-transferrin-sodium selenite (ITS) media supplement, sodium pyruvate, and penicillin-streptomycin were purchased from Invitrogen (Grand Island, NY).
It is also used in cell culture media supplements Sodium Cholate, Pierce Biotechnology, USA.
The dissolution media were sodium phosphate buffer, pH 7.0, and ammonium acetate buffer, pH 6.8.
In conclusion, TDF-SD wasuccessfullyly improved in vitro dissolution rate of TDF compared to commercial products (Cialis®) in the dissolution media without sodium lauryl sulfate (SLS).
The sole use of commercial media containing sodium polyanethol sulfonate may render the bacterial culture negative.
RPE cells were maintained in either MEM or high-glucose DMEM, both media without sodium pyruvate, plus 10% FBS and C2C12 cells were cultured in high-glucose DMEM without pyruvate plus 10% FBS.
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