That hasn't stopped a series of properly idiotic attempts at media seeding, with Boyle herself suggesting Meryl Streep was seriously looking at the role.
Pots were placed into racks in the greenhouse, flooded with water to settle soil media, seeded and covered with plastic wrap to maintain a humid environment.
Cells of interest were washed with media, seeded in fresh media into 96-well plates at a density of 50 × 10 cells/well, treated with appropriate agents and incubated at 37 °C with 5% CO2.
The media was seeded with 10 mL prepared inocula.
C. elegans animals were maintained at 20°C on standard nematode growth media plates seeded with E. coli OP50 bacteria.
Vented 225 cm tissue culture flasks containing R10+ media were seeded with 3 4 x 10 alveolar Mϕ and placed in a humidified 37°C incubator containing 5% CO2.
Cells were washed twice with culture media and seeded with or without SEVC4 cells at a ratio of 1 1 (2 × 10 cells of each cell type/well in 96-well plates).
Re-feeding was performed by washing animals from starvation plates with M9 + ~0.5%OP500, spinning at 100 200g for ~1 min to pellet animals, and then depositing animals onto 10-cm nematode growth media plates seeded with OP50.
