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These experiments were done in either single and co-cultures in complete media or in conditioned media of single and co-cultures.
Single glioma spheroids were placed in the center of each well of a 0.5% agarose-coated 48-well microplate and 200 µL of conditioned media of single cultures and co-cultures with hUCBSC was added to each well.
This protein was undetectable in cell layers or media of single cultures of MSCs, but clearly detectable in co-cultures.
Since our previous assays support an important contribution for soluble factors to create an inflammatory protumoral microenvironment, we used MCF-10A cells as sensors of protumoral factors present in media of single and cocultured cells.
To characterize the paracrine factors secreted in this model system, the concentrations of transforming growth factor β1 (TGF-β1), matrix metalloproteinase 2 (MMP2) and monocyte chemoattractant protein-1 (MCP-1) were determined in the media of single component cultures and cocultures.
Similar(55)
Embryogenic suspensor mass (ESM) cultured continuously on proliferation media consisted of single early somatic embryos, free suspensor cells and polyembryogenic complexes.
The model adopted radiative flux computation method to calculate radiative heat transfer between fluidized disperse phase contacting to the wall and immersed walls, in which the absorption and back-scattering coefficients was obtained from the reflectivity and the absorptivity of a layer of disperse media of a single particle thickness.
Diaz points out a frustrating truth about mainstream media's depiction of single women: the assumption that no woman chooses to be on her own.
Removal efficiency of mixed media for pollutants was higher than that of single media (ZVI only).
48 h before phenotyping, frozen strains were thawed and 10 μl of cells were inoculated into 500 μl of YEPD media, or single colonies of individual engineered yeast strains were inoculated into 5 ml YEPD media + 200 μg/ml Geneticin, in the interior wells of a 96-deep well block (NUNC) with a multichannel pipettor.
Incubation of CD34−CD11c+ immature DCs with tumour cell-conditioned media led to progressive alignment of single cells oriented with the same polarity, forming string-like structures.
Related(19)
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