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All strains exhibited similar growth characteristics when grown overnight in epithelial cell media (not shown).
Similar results were seen whether cells were sorted into serum-containing or defined bFGF/EGF neurosphere media (not shown).
Mammospheres can be generated by single cells, as we also observed in both FCS and GFs-supplemented media (not shown), and yet display a heterogenic progenitor composition [ 22].
In control experiments, moss colonies treated with either Luria-Bertani (LB), potato dextrose broth (PBD) growth media (not shown) or with spores of a non-pathogen, Aspergillus nidulans (not shown), did not develop disease symptoms.
We constructed various Δ adhE derivatives of both FTD147 and JW135 (FTJWDC3FTJWGH1, JWGH1, DG2, FTDPH10) and verified that they were unable to grow under anaerobic conditions on sorbitol or glucose minimal media (not shown).
Fibroblast cultures obtained from the father (I-1) fulfilled criteria of senescence from the beginning, as they did not show population doublings within 4 weeks despite frequent changes of cell culture media (not shown).
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First, to rule out the effect of CSC media on the expression, monolayer cells grown in the CSC media were checked, and no difference was found between cells grow in CSC media and regular growth media (data not shown).
MF201, MF213 and MF214 exhibited no growth defects either in liquid media (Figure S1) or on solid media (data not shown).
During our independent experiments, we found that iPS induction in FBS based media was slower than in KSR based media (data not shown), consistent with recent report (Okada et al., 2010).
This requirement was not observed for flo10 Δ strains grown in minimal media (data not shown); thus, this effect is very likely dependent on media composition.
To further confirm removal of KanMX4 module, we tested these cells on G418 selection media and did not find the cells grew on G418 media (data not shown).
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