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miR-21 expression in media, normalized to cellular proliferation did not significantly influence these relationships (supplementary Fig. 3).
After 3 weeks, clusters of cells growing in wells were appreciable and the IgG concentration in the clone supernatants was 10 40 μg/ml/106 cells, as determined by means of an immunoenzymatic method, measuring IgG concentrations in the media normalized for cell densities.
GAG released to media (normalized to DNA content): No significant differences in GAG released to media were observed between media groups (data not shown).
For rescue assays CUY1147ΔLEU2 transformed with rescue plasmid or empty vector was grown overnight in CSM-Leu media, normalized to A600 = 1, and plated at serial 10-fold dilutions onto CSM-Leu plates with or without 500 μM CuSO4.
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The media have "normalized" the grotesquely abnormal.
Lyophilized conditioned media were normalized to the number of cells mixed with non-reducing LaemmLi buffer and subjected into 10% SDS-polyacrylamide gels containing 1 mg/mL gelatine in the presence or absence of BP.
LDH release into media was normalized to total LDH release for each well.
There's also the internal danger that the media will normalize his time in office under the cloak of traditional reverence for the presidency.
Dating back to Roland Barthes and his writings on myth, semiotics at its best is an instrument against power, a way to reveal how mainstream media narratives normalize and perpetuate an unhealthy, unjust status quo.
D. Calculate the effect of PLX4720 in the presence or absence of conditioned media by normalizing the number of cells after 7 days of treatment (as measured by GFP fluorescence) to the number of cells present in the SK-MEL-5 vehicle control condition.
The volumes of the conditioned media were also normalized by cell numbers so that an equal volume of FCM or PCM was produced by equal number of FOb or POb cells.
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CEO of Professional Science Editing for Scientists @ prosciediting.com