All strains were initially evaluated by their ability to grow on YEM agar media modified by the addition of PCNB, named P-YEM.
Each consortium was inoculated (1% inoculum load) in 50 ml synthetic media: modified Czapek mineral-salt broth [(g/l) sodium nitrate 2, potassium chloride 0.5, magnesium sulphate 0.5, di-potassium hydrogen phosphate 1, ferrous sulphate 0.01 supplemented with CMC, pectin, starch, skimmed milk and tributyrin (0.5% each)] and incubated at 37 °C at 150 rpm for 7 days (Sarkar et al. 2011).
In the heterogeneous environment, the five test tubes were randomly assigned to growth media modified to 4∶1, 8∶1, 16∶1, 32∶1, and 64∶1 ratios of NO3− to PO43−, respectively.
All cultures were grown at 37°C in well-mixed minimal media modified with M63 (pH 7.0, 62 mM K2HPO4, 39 mM KH2PO4, 15 mM ammonium sulfate, 1.8 µM FeSO4-7H2O, 15 µM thiamine hydrochloride, 0.2 mM MgSO4-7H2O and 22 mM glucose; mM63 [56]).
All samples, other than stool samples, were collected in virus transport media modified according to recommendations by the World Health Organization (12 ).
The dish was filled with either "EGL collapsing" media (Modified KREBS-Henseleit buffer + 1% BSA) or "EGL preserving" media (incubation medium).
H3515 MIAMI cells were seeded at 500-800 cells/cm in MIAMI media, modified to contain 10% FBS (to boost overnight proliferation).
In Figure 3, the studies are chronologically ordered, whereas in Figure 4 their order is based on the proportion of children with bilateral acute otitis media (modified forest plot).
In order to evaluate the effect of nitrate stress on Blackbeard mRNA levels, cells were transferred to media modified with 50 μM NO3- and maintained in exponential phase in semi-continuous batch cultures.
Efe et al. [ 18] reported that addition of a small molecule Janus kinase (JAK) inhibitor in mouse embryonic fibroblasts (MEFs) transiently expressing OSKM factors in reprogramming media modified to hinder iPSC induction and promote cardiomyogenesis resulted in formation of spontaneously contracting cardiomyocyte colonies.
