Exact(2)
A high degree of lipid accumulation was observed in several of the strains although no special treatments such as nitrogen-free media inoculation or UV mutagenesis was applied.
Optical microcopy was used for periodical examination of crystal presence in the media up to the 30 days after media inoculation.
Similar(58)
Samples and media preparation, inoculation and colony counts were done using standard microbiological methods (Buckle et al. 1989; Bartram and Balance 1996; FAO 2004).
The capability of the Sharklet micropattern to reduce S. epidermidis colonization was independent of conditions, i.e. pre-treatment media and inoculation duration, based on a Tukey simultaneous test to evaluate significant differences among log reductions (Table 1).
Sodium acetate (20 mM final conc)., sodium formate (60 mM final conc)., coenzyme M (10 µM final conc)., and vitamin-supplemented clarified rumen fluid were added to sterile media, before inoculation with 0.5 ml of an actively growing culture of M. ruminantium, then gassed with H2 plus CO2 (4∶1) to 180 kPa overpressure.
Media without inoculation was used as a control for abiotic oxidation.
When needed, l-cystine (Sigma) was added to the media from inoculation at a final concentration of 5 mM (l-cystine was dissolved in 1 M HCl at a concentration of 5% w/v before addition to the growth media).
To evaluate recovery of M. bovis, recovery of atypical mycobacteria and contamination rates for each media, each inoculation protocol (liquid only, low risk- histologically negative, and high risk or histologically positive) was evaluated separately using log linear models to obtain point estimates and 95% confidence intervals with R software.
The media were inoculated with 0.4 mL of a standardized bacterial inoculation.
Fungal culture results were considered positive when there was growth of the same organism on two or more solid media at the inoculation site, or when the organism grew on one culture media and was noted on a stained smear (gram, Giemsa, or Gomori methenamine silver) [11].
Sodium benzoate was added to the media prior to inoculation at a concentration of 30 mM.
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