Sentence examples for media grown for from inspiring English sources

Briefly, cells were seeded at 106 per ml in MEM-pros media, grown for ∼64 hours with gentle rocking and then diluted 1∶5 in fresh MEM-pros medium, auto-conditioned as described previously [55].

S. cerevisiae strains were transferred from cryo-preserved cultures to SC solid media, grown for 2 days and selected on SM solid media, supplemented only with required amino acids/ nucleobases for 1 day.

For production of recombinant proteins, bacteria were then diluted 1 100 in LB + amp media, grown for 2 hrs and induced with 0.2 mM IPTG for 2 4 hrs.

MVt-ΔH1 was produced using 500 ml of auto-induction media grown at 30°C for 20 hr in presence of 40 mg/l of kanamycin [31].

Two microliters of the aforementioned serial diluted yeast cells were spotted onto SD-LT, SD-LTH + 3-AT and SC-LTHA media, grown at 30°C for 2 d (SD-LT plates) or 5 d (selective plates) before photographed.

Two microliters of the aforementioned serial diluted yeast cells were spotted onto SD-LW, SD-LWH+3-AT and SD-LWHA media, grown at 30°C for 2 d (SD-LW plates) or 7 d (SD-LWH+3-AT and SD-LWHA plates) before photographed.

Two microliters of the aforementioned serial diluted yeast cells were spotted onto SD-LT, SD-LTH + 3'AT and SD-LTHA media, grown in 30C for 2-5 days, before photographed.

Following collection of a high-Pi sample, cells were washed three times in no-Pi media, transferred to no-Pi media, and grown for either 60 or 120 minutes.

Cells were grown at 30° to saturation in liquid YPAD media, diluted 1 1000 in fresh YPAD media and grown for 18 hr at 30° in a microplate reader (Sunrise model, Tecan).

Yeast cells expressing eGFP or RFP fusions were grown in synthetic complete media to stationary phase, then diluted 1 20 into synthetic complete media and grown for 6 hr with shaking.

At confluence, media was exchanged for DMEM-F12 or F-12 serum-free media and grown for 3 4 days before collection.