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Accordingly, conditioned media generated from in vitro stimulated and non-stimulated spleen cell cultures were assessed in cytokine bead array assays.
Both assays revealed that only the conditioned media generated from WT cells accelerated the migration of BMSCs, while the conditioned media from IL-6 KO cells was unable to elicit the same effect.
Plating efficiency was determined by plating the cell lines at a known density (20 000–30 000 cells per dish) in conditioned media generated from the RM of cells that had been growing exponentially in a 9 cm diameter plate for 3 days.
We hypothesize that NCCM generated from NCs in their native tissue environment will trigger differentiation of MSCs toward an NP phenotype to a greater extent than both notochordal media generated from NC cells in alginate and chondrogenic media (TGFβ-3) alone.
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Rachel Taylor, an artist, says she wants to make a dress from the social media generated by London Fashion Week.
The media generated a lot of their hype from the idea that young people had "come out for the event", toadying royal correspondents using a couple of kids flapping Union Jacks around to justify the role of the Royal family in modern Britain.
Representatives from governmental and non-governmental groups and the media generated considerable dialogue.
I feel responsible for murder and slaughter that's taken place in South Sudan that's not being reported on, partly because there's not as much social media being generated from there.
Conditioned media were generated from cultured IFP and scAT explants, and cells (chondrocytes, synoviocytes) were isolated after sequential enzymatic digestion of the corresponding tissues.
"Randy didn't pick me because he likes me personally, he picked me because of the media I generated from the Indy win," Wheldon confirmed.
Our study showed a paucity of policy and media briefs generated from most SPHs.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com